4t1 cells p53. May 23, 2018 · Expression of p53 repressed γ-H2AX.
4t1 cells p53. They The ability of mutp53 to drive cancer cell migration and invasion was further confirmed by forced expression of p53 R172H and p53 R270H in 4T1 cells (Figures 2A and 2B). All cells used in this study were authenticated using short tandem repeat (STR) profiling and propagated in the recommended serum-supplemented medium. We report a mechanism by which mtp53 suppresses both cell-autonomous and non-cell-autonomous signaling to promote cancer cell survival and evasion of tumor immune surveillance. The 4T1 murine mammary cancer cell line is one of the most widely used breast cancer models. This study explores if low-dose statins can curb mutant p53 activation in breast cancer’s immune signaling, hindering tumor immune evasion. The expression of pro-apoptotic and anti-apoptotic genes in both cell lines was determined by measuring mRNA levels using real-time polymerase chain reaction (PCR). PRIZE ensures precise delivery to tumor sites Feb 9, 2015 · This study identified 14-3-3ζ as a molecular switch turning TGF-β from tumor suppressor to metastasis promoter by altering Smad partners from p53 in premalignant cells to Gli2 in cancer cells. Nov 22, 2022 · P53, BAX, BCL-2 in MCF-7 cells, B. Biomed Pharmacother. Methods: The study used diverse breast cancer cell lines with varying p53 statuses. TP53 mutations occur in many different types of cancers that produce mutant p53 proteins. Jan 2, 2020 · Background The clinical success of immune checkpoint inhibitors demonstrates that reactivation of the human immune system delivers durable responses for some patients and represents an exciting approach for cancer treatment. 001). Aug 1, 2018 · SGT-53 increases immunogenicity of 4T1 cells Following exposure of 4T1 mouse breast cancer cells in culture to a tumor-targeting nanocomplex loaded with a plasmid encoding human wtp53 (SGT-53) or with an empty vector control plasmid (scL-vec), quantitative RT-PCR was performed to assess expression of human p53 (Figure 1A) as well as mouse genes associated with immune responses (Figure 1B). P460 is a wild-type peptide derived from the p53 C-terminus and has been proven to be capable of restoring the tumor suppressor function of p53. Here, PRIZE, a P53‐repair nanosystem based on a virus‐mimicking nanostructure to deliver p53 mRNA and Zn (II) into tumor cells, domesticating tumor cells by restoring intracellular P53 levels to bolster their immunogenicity, is designed. Apr 1, 2023 · To investigate the effects of p53 WT and CGAS interactions on anticancer immune responses, Ghosh and colleagues compared the growth of WT versus Cgas–/– or Sting1–/– 4T1 cells optionally driven into p53 WT overexpression after implantation into immunocompetent syngeneic mice. 9. C. It lowered mitochondrial transmembrane potential and induced mitochondrial fragmentation. 001 and P < . For enhancing the MCF7 cells (established from human Luminal A, ER+, PR+, HER2- breast cancer [60]) express high levels of the epithelial cell marker E-cadherin (also known as Cadherin-1). Its soluble form is induced by secretagogues (e. Mutant p53 (mtp53) proteins can exert cancer-promoting gain-of-function activities. Oct 1, 2025 · 4T1 breast cancer cells exposed to extracellular vesicles from MDA-MB-231 cells stimulated with Bisphenol A increase the growth of mammary tumors and metastasis in female Balb/cJ mice Oct 1, 2025 · 4T1 breast cancer cells exposed to extracellular vesicles from MDA-MB-231 cells stimulated with Bisphenol A increase the growth of mammary tumors and metastasis in female Balb/cJ mice Jan 28, 2022 · DEA reduced the viability, colony formation, and invasive growth of the 4T1 cell line and led to a higher extent of the MCF-7 cell line. Apr 12, 2021 · Summary Mutant p53 (mtp53) proteins can exert cancer-promoting gain-of-function activities. For in vivo studies, BALB/c tumor-bearing mice were used to evaluate the inhibitory effect of Gemini-Cur in comparison with mice . 4T1 metastasis examination 4T1 cells were cultured in completed RPMI 1640 media, at 37°C in a humidified incubator in 5% CO 2. Methods Breast cancer 4T1 cells were divided into 6 groups: control, brazilin 1/2 half maximal inhibitory concentration (IC50), IC50, 2×IC50, erastin (10 µg/mL) and capecitabine (10 µg/mL) groups. Different p53 mutations SUMMARY Mutant p53 (mtp53) proteins can exert cancer-promoting gain-of-function activities. Jan 1, 2023 · Cells The human osteosarcoma cell line Saos2, breast cancer cell lines MCF-7, SK-BR-3, MDA-MB-468, and BT-549, and mouse breast cancer cell line 4T1 were obtained from ATCC (Manassas, VA, USA). g. PRIZE ensures precise delivery to tumor sites Mar 1, 2015 · Results were compared to non-metastatic 67NR and metastatic 4T1. 2016; 84:637-644 (ISSN: 1950-6007) Delphi L; Sepehri H PURPOSE: Increase in the number of cancer related deaths has made the study on developing new drugs and treatments essential. Although 4T1 cells lacked detectable expression of p53, those engineered to express CST-Abl exhibited robust production and secretion of TGF-β1 that engendered the reactivated expression of p53. (B) The mRNA expression of CPT1C is upregulated by p53 hot-spot Enhanced transfection kit designed for superior efficiency in the 4T1 cell line, a model of breast cancer that is widely used in biomedical research. New information regarding the involvement of innate and acquired immunity in Feb 18, 2025 · The efficacy of in situ cancer vaccines (ISCVs) is hindered by the poor immunogenicity of tumor cells. Aug 1, 2019 · More specifically, we characterized cell morphology, cell-cell interactions, polarity and motility of mouse tumor cell lines 4T1 and mILC-1 and human tumor cell lines MDA-MB-231 and T47D. from publication: Immunization May 23, 2018 · Expression of p53 repressed γ-H2AX. A The studied mammary tumour arose from 4T1 cells with non-functional p53 and zinc transporters set to zinc accumulation (13), thus, the hypothesis about the toxicity of excessive zinc for the tumour tissue was tested. In this study, we examined oridonin's effects on 4T1, MCF-7, and MDAMB-231 cellular activity using CCK8. Then tumor volumes, histological analysis and immunohistochemical staining of P53 and tunnel test were applied to evaluate apoptosis in tumors. [1]. 05, respectively) and caspase-3 genes (P < . (A) Immunoblots show p53 null 4T1 cells infected with PLVX or p53R249S expression vectors. More-over, p53 knockdown with two different shRNAs reduced TBK1 substrate phosphorylation in CT26 murine colorectal can-cer cells (Figure 1D). Jul 23, 2020 · The 4T1 murine mammary cancer cell line is one of the most widely used breast cancer models. The studied mammary tumour arose from 4T1 cells with non-functional p53 and zinc transporters set to zinc accumulation (13), thus, the hypothesis about the toxicity of excessive zinc for the tumour tissue was tested. (Fig. Effects of oridonin on the cell cycle and cycle-related proteins of 4T1 cells. Protein and transcript levels of markers of human breast cancer molecular subtypes were measured in the four tumour lines, as well as p53 (Tp53) tumour-suppressor gene status and responses to tamoxifen in vivo and in vitro. 5 E) revealed that the downregulation of DLGAP5 in MDA-MB-231 and 4T1 cells markedly upregulated p21 expression and the p-p53/p53 ratio, suggesting that DLGAP5 knockdown could activate the p53 pathway in TNBC cells, potentially inhibiting the malignant phenotype of TNBC. Almost all measured parameters were remarkably heterogeneous even between positions within the same tumor. Here we describe two 4T1 cell lines modified to facilitate analysis of tumor growth and metastasis and evaluation of gene function in vivo. Feb 18, 2025 · The efficacy of in situ cancer vaccines (ISCVs) is hindered by the poor immunogenicity of tumor cells. 5 ng/ml CIS for 18 hours prior to use as targets in various assays. Graphical representation of the cell cycle distribution of 4T1 cells after treatment with oridonin. A and B. show that p53 mutant cancers express high levels of the Base Excision Repair (BER) pathway and that deoxyuridine analogues induce DNA damage in p53-mutant TNBC cells. 05, respectively) and the expression of p53 (P < . In this study, we explored whether WAT browning occurs in murine breast cancer, and sought to answer if E0771 or 4T1 cells, well-characterized murine breast cancer cell lines, or IL6 are potential drivers of WAT browning in an in vitro cell culture system. In the presence of T cells, the combined p53 and Ps therapy exhibited superior antitumor efficacy compared with either treatment alone. Jan 19, 2023 · We engineered p53 null 4T1 breast cancer cell line to inducibly express WTp53 and we observed that WTp53 expression increased TBK1 substrate phosphorylation (Figure 1 C). Apr 10, 2019 · nt. CT-26, Vero or 4T1 cells were irradiated with UV at the indicated doses to induce DNA damage and tested 16 h post-irradiation for expression We report that transfection of 4T1 mammary tumor cells (p53-null) with the dendritic cell (DC) growth factor, fms-like tyrosine kinase 3 ligand (Flt3L), significantly delayed their growth in vivo, resulting in the rejection of 100% of the tumors formed by injection of tumor cells cotransfected with Flt3L and p53. 01) in the PR and TM groups. Here we report a selective combination therapy strategy for treatment of p53 mutant cancers. Jul 1, 2023 · Oridonin's effects on the breast cancer cell cycle were studied using flow cytometry, and expression of cell cycle related proteins p53, CDK2, and p21 was detected using Western blot assays. Jan 2, 2024 · Here the authors show that gain-of-function mutant p53s predispose cells to chromosomal instability by targeting MCMs, leading to activation of a cGAS-STING-non-canonical NF-κB signaling that Apr 4, 2012 · In this study, we examined the mechanism of apoptosis in p53- null breast cancer cells in response to the proteasome inhibitor bortezomib. Similar results were obtained in 4T1 cells (harboring p53 P31Ter, an early truncation, and is effectively a p53-null line) transfected with WTp53, p53 R172H, or p53 R270H (Figure 1 G). May 26, 2022 · Comparison of MEFs derived from p53 wildtype (p53+/+) or null (p53-/-) mice showed a clear positive correlation between WTp53 and cGAS/STING pathway. Increased expression of phosphorylated p53 and p53 and decreased expression of Bcl-2 were found in Tan IIA-treated 4T1 cells. (A) The differential expression of lipid metabolism-related genes induced by mutant p53. 4T1 cells, derived from a murine mammary carcinoma [61], exhibit epithelial morphology, E-cadherin expression, and also some degree of vimentin positivity. The expression of p53 in 4T1/p53 transfectant (clone 1) was confirmed by a Western blot analysis with Ab-7 using a p53 -positive cell line (MDA-MB-468) as a control. a Representative dot plots of EpCAM expression on 4T1 and MDA-MB-231 breast cancer cells and JAWSII, normal non-cancerous dendritic cells Apr 1, 2023 · To investigate the effects of p53 WT and CGAS interactions on anticancer immune responses, Ghosh and colleagues compared the growth of WT versus Cgas–/– or Sting1–/– 4T1 cells optionally driven into p53 WT overexpression after implantation into immunocompetent syngeneic mice. An important class of preclinical in vivo models for immuno-oncology is immunocompetent mice bearing mouse syngeneic tumors. Metastasis was detected using an in vivo imaging system (IVIS; Caliper) by blocking the signal from primary xenografts. T cells in tumor tissues and spleens were analyzed, and the in vivo biosafety of the Pos3Aa-p53 crystal/anti-PD-1 antibody combination was also evaluated. Finally, both unilateral and bilateral 4T1 tumor mouse models were utilized to assess the efficacy of Pos3Aa-p53 crystal-mediated p53 restoration in enhancing the antitumor activity of ICIs. d – m Background: Tumor models are critical for our understanding of cancer and the development of cancer therapeutics. Cells were inoculated in the left flank area of female nude mice at a concentration of 1×10 6 /mouse. Conclusion: Our findings demonstrated that chlorogenic acid plays a notable role on apoptosis inducing in the 4T1 cells through regulation of apoptotic proteins. p53 WT expression limited the growth of 4T1 cells, an effect Breast cancer is one of the most common cancers. 5 nM TAX, 12. To facilitate translation of preclinical Dec 1, 2016 · MTT cell proliferation assays, double fluorescence staining (acridine orange/ethidium bromide) and cell cycle analysis were employed to measure apoptosis in vitro. Materials and Methods: In this study, cell proliferation was measured using an 3-(4,5-methylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay (MTT) on 4T1 cells. Results Delivery of p53 protein into p53-null TNBC 4T1 cells via Pos3Aa-p53 crystals restored the p53 activity, and therefore led to the induction of ICD, activation of type I IFN signaling In this study, we examined the mechanism of apoptosis in p53-null breast cancer cells in response to the proteasome inhibitor bortezomib. Feb 18, 2025 · This research highlights that PRIZE can efficiently repair P53 abnormalities in 4T1 (P53‐deficient) and MC38 (P53‐mutant) cells, subsequently upregulating the expression of major Jun 5, 2025 · TP53 inactivation in human cancers often results from MDM2/MDMX overexpression, yet therapeutic targeting remains challenging owing to incomplete mechanistic understanding. Moreover, p53 knockdown with two different shRNAs reduced TBK1 substrate phosphorylation in CT26 murine colorectal cancer cells (Figure 1 D). Background/Aim: Prostate apoptosis response 4 (PAR4), a tumour-suppressor protein, selectively induces apoptosis of cancer cells without affecting normal cells. Using diverse tumor models including p53−/− genetic mouse model and syngeneic tumor models, we identified primordial germ cell (PGC)-like tumor cells, which are enriched in earliest liver micro-metastasis (up to 99%), as a cell origin of liver metastasis. METHODS:Molecular docking of esculetin with p53, solute carrier family 7 member 11(SLC7A11)and glutathione peroxidase 4(GPX4) proteins was performed, The expression level of P53 and caspase-3 increased during treatment with chlorogenic acid in the 4T1 cells (P < 0. High-fat diet (HFD)-induced LD accumulation in tumor cells elevates LD-surface MDM2 through Cyb5r3-Myh9 interactions, which We would like to show you a description here but the site won’t allow us. Afterwards, other assays like P53, Caspase-3 proteins expression and Annexin V/PI were detected by flow cytometry. tigate the mechanism through which esculetin induces ferroptosis of mouse breast cancer 4T1 cells. 5 ng/ml DOX, and 12. Also; Bax and Bcl-2 were May 2, 2025 · Mutations in the critical TP53 gene, which normally prevents cancer, can instead promote it. Sep 1, 2017 · A 100-fold differential of EpCAM expression on TNBC cells. Mar 24, 2020 · Of note, Stellarhigh PGC-like tumor cells, but not Stellarlow non-PGC-like cells, sorted from primary tumors of p53−/− mice readily form liver metastasis. Here, we present an integrated map of the genome, transcriptome, and immunome of 4T1. p53 WT expression limited the growth of 4T1 cells, an effect T cells in tumor tissues and spleens were analyzed, and the in vivo biosafety of the Pos3Aa-p53 crystal/anti-PD-1 antibody combination was also evaluated. The results of real-time RT-PCR showed that CGA therapy increased the expression ratio of Bax/Bcl-2 (P < . Jan 28, 2005 · Challenge with Cl-66 revealed that immunization with irradiated 4T1-Flt3L-p53 cells significantly slowed growth, prolonged survival, and resulted in complete remissions. Mutant p53 activates FAO activity through upregulating CPT1C. Other frequently mutated genes in breast cancer, including Brca1 and Brca2, are not mutated. Jan 19, 2023 · We engineered p53 null 4T1 breast cancer cell line to inducibly express WTp53 and we observed that WTp53 expression increased TBK1 substrate phosphorylation (Figure 1C). 1A) We engineered p53 null 4T1 breast cancer cell line to inducibly express WTp53 and we observed that WTp53 expression increased TBK1 substrate phosphorylation. Aug 9, 2008 · Background The 4T1 mouse mammary tumor cell line is one of only a few breast cancer models with the capacity to metastasize efficiently to sites affected in human breast cancer. Mar 15, 2010 · Breast cancer cell lines TUBO, 4T1, and 4T1-Neu (a stably transfected tumor cell line that expresses Neu antigens) were described previously Tumor cell lines were treated with 12. The mutant p53 proteins have lost wild-type p53 activity and gained new functions that contribute to malignant tumor progression. One of the main Feb 11, 2020 · We thank Sven Rottenberg for providing us tumor pieces from K14cre; Brca1 F/F; p53 F/F mice; Ágnes Csiszár for the 4T1 cell line, Irén Bodrogi-Mayer, Anita Hídvégi and Annamária Tóth for excellent technical assistance. This opens new possibilities to the synergistic delivery of gene drugs in conjunction with epigenetic therapy strategies to treat both genetic diseases and cancer. Representative images (left) and quantification (right) of the invasive ability. The relative mRNA expression level of ACSM3, ACSL4, CPT1A, CPT1B, CPT1C, and SLC25A20 between control and MCF12A-p53 R280K cells were determined by Q-PCR (mean ± SD, n = 3). Western blot analysis of the expression of the S phase arrest-related proteins p53, p-p53, p21 and CDK2. Scratch assays were used to detect oridonin's effects on cellular migration. We report a mechanism by which mtp53 suppresses both cell-autonomous and non-cell autonomous signaling to promote cancer cell survival and evasion of tumor immune The evaluation by H&E staining showed cell apoptosis in both the PR and TM groups. Results were compared to non-metastatic 67NR and metastatic 4T1. MTT and annexin V/FITC assays were performed to study the toxic effect of Gemini-Cur on mouse cancer cells. We also observed that AKR1B1 overexpression promoted metastasis in the 4T1 orthotopic model of triple-negative breast cancer. Metabolomics assays were used to detect changes in small molecule metabolites and metabolic pathways in breast cancer cells after treatment with oridonin. p53 WT expression limited the growth of 4T1 cells, an effect Experimental design: MTT cell proliferation assays, double fluorescence staining (acridine orange/ethidium bromide) and cell cycle analysis were employed to measure apoptosis in vitro. Jul 12, 2021 · Zonneville et al. Western blot analysis of p53 expression and function. p53, Bax, Bcl-2 in 4T1 cells. Two breast cancer cell lines: MDA-MB-231 (mutant p53) and 4T1 (null p53), two colon cancer cell lines: HCT-116 (wild-type p53) and CT26. When cells suffer amino acids scarcity, mutant p53 protein is stabilized and induces metabolic alterations and an amino acid transcrip-tional program that sustain cancer cell proliferation. MDM2 controls CD8 + T cell-mediated anti-tumor immunity Because MDM2 interacts with p53 and regulates p53 stability and activity, targeting the p53 and MDM2 interaction offers a potential therapeutic approach through reactivation of p53 in tumor cells retaining wild-type p53 status 2. Results: We found Trp53 (Tp53) and Pik3g to be mutated. Apr 24, 2024 · Brazilin actuated ferroptosis in breast cancer cells, and the underlying mechanism is mainly associated with the p53/SLC7A11/GPX4 signaling pathway. May 1, 2025 · When T cells were added to 4T1 cell cultures, a significantly enhanced clearance of 4T1 cells was observed in groups expressing Ps. The effect of brazilin on the proliferation of 4T1 cells was detected by cell counting kit-8 Western blot analysis (Fig. (B) Invasion assay of shCtrl and shRCP (#1, #2) 4T1 cells allowed to invade into Matrigel for 72 hr, n=5. 2 tumours of the 4T1 model. The aim of the current study is to compare the cytotoxic effects of the most commonly used statins: atorvastatin (Lipitor®) and simvastatin (Zocor®) on a variety of cancer cells with different p53 status. As 4T1 is a common model for metastatic tumors, our data supports the rational design of mode-of-action studies for pre-clinical evaluation of targeted immunotherapies. May 8, 2025 · To eliminate the direct impact of cancer cells on CD8 + T cells, we isolated CD8 + T cells from mouse spleen (Figures S7 G and S7H); co-cultured them with WT, DEL, H355A, and DEL H355A 4T1 cells; and analyzed them by western blot analysis (Figure 5 C). Jan 15, 2015 · In order to further delineate the mechanism of p53-independent induction of apoptosis after proteasomal inhibition, we examined the differential expression of proteins in response to bortezomib-treatment by proteomic techniques in p53-deficient 4T1 breast cancer cell, which is commonly used for in vivo breast cancer tumor models. Feb 7, 2020 · a – c Parental 4T1 cells and D0–D25 sublines were subjected to microchip analysis and heat map for the 194 transcripts involved in cell cycle and p53 signaling pathway was constructed. Apr 12, 2021 · We also engineered the 4T1 mouse breast cancer cell line (p53 null) to express the R249S mtp53 and found that these cells had reduced TBK1 substrate phosphorylation (Figure 1D). The efficacy of in situ cancer vaccines (ISCVs) is hindered by the poor immunogenicity of tumor cells. Aug 11, 2024 · We developed a biomimetic liposome gene delivery technology to integrate an epigenetic regulator, p53, and immune checkpoint modulation for enhancing tumor therapeutic effect. Mar 1, 2023 · Fluorescence microscopy was employed to visualize cellular uptake and morphological changes of 4T1 cells during treatment with Gemini-Cur and void curcumin. The poor accumulation of drugs in tumors is a serious hindrance to tumor treatment. Jul 7, 2020 · The ability of mutp53 to drive cancer cell migration and invasion was further confirmed by forced expression of p53 R172H and p53 R270H in 4T1 cells (Figures 2 A and 2B). Sep 14, 2023 · In cancer cell lines, p53 reduced AKR1B1 mRNA and protein levels and repressed promoter activity in luciferase assays. Jan 30, 2019 · The 4T1/BT549-nmrHas2 cells could secrete EHMW-HA (with a molecular weight of up to 6 MDa), which was similar to that found in the naked mole rat. WT (null p53), and two bladder cancer cell lines Dec 25, 2023 · The restoration of the function of p53 in tumors is a therapeutic strategy for the highly frequent mutation of the TP53 tumor suppressor gene. Representative graphical quantification of in vitro cell proliferation between 4T1 PLVX and p53R249S cohorts. , chloroquine), and it induces apoptosis by interacting with the receptor of glucose-regulated protein 78, which is overexpressed in cancer cells. View This Abstract Online Apple pectin: A natural source for cancer suppression in 4T1 breast cancer cells in vitro and express p53 in mouse bearing 4T1 cancer tumors, in vivo. Oridonin, a traditional Chinese medicine, is believed to inhibit tumor growth, but its particular effects on breast cancer remain unknown. Here, we present an integrated map of the SUMMARY Mutant p53 (mtp53) proteins can exert cancer-promoting gain-of-function activities. Initially, we determined the p53 status of 4T1 breast carcinoma and 4THMpc (a highly mestatic derivative of 4T1) cells and verified that both cells are p53 deficient. a Lysates prepared from 4T1 cells were subject to Western blotting and immunoprecipitation with antibodies against H2AX and p53. Conclusions: 4T1 cells share substantial molecular features with human TNBC. Here, PRIZE, a P53-repair nanosystem based on a virus-mimicking nanostructure to deliver p53 mRNA and Zn (II) into tumor cells, domesticating tumor cells by restoring intracellular P53 levels to bolster their immunogenicity, is designed. Dec 1, 2014 · This data article contains data related to the research article entitled, “A proteomic analysis of p53-independent induction of apoptosis by bortezomib in 4T1 breast cancer cell line” by Yerlikaya et al. Hence, this study evaluates chlorogenic acid effects on 4T1 breast cancer cells. RT-PCR was performed on 4T1 cells transfected with hFlt3L- and p53-specific primers as described in Materials and methods. In this study, curcumin was analyzed 2. Oct 25, 2023 · Mutant p53 promotes serine and glycine synthesis and intake of essential AAs in BC cells To investigate whether mutp53 may impact de novo AAs synthesis, we performed a metabolic tracing experiment Breast carcinomas commonly carry mutations in the tumor suppressor p53, although therapeutic efforts to target mutant p53 have previously been unfruitful. Apr 23, 2024 · Objective To investigate the mechanism of induction of ferroptosis by brazilin in breast cancer cells. Since we have previously shown that 4T1 cells are p53‐null cells (5), the present results indicate that these anticancer Jan 1, 2023 · For spontaneous metastasis assays, cells of the mouse breast cancer cell line 4T1-luciferase (1 × 10 5) were orthotopically injected into the mammary fat pads of mice and incubated for one month. Lipid droplet (LD) enrichment is identified as a key trigger for MDM2-mediated p53 degradation. Relative expression levels of p53, p-p53, p21 and CDK2 proteins (ns, no significant difference,**P 16), which are comparable to the values obtained in this study with the 4T1 breast cancer cells. Jul 19, 2025 · Our results suggest that OBP-702-mediated presentation of p53 epitopes on tumor cells enhances the antitumor efficacy of Ad-p53 DCs against murine CC tumors by attracting p53-targeting CTLs. To investigate the effects of p53 WT and CGAS interactions on anticancer immune responses, Ghosh and colleagues compared the growth of WT versus Cgas–/– or Sting1–/– 4T1 cells optionally driven into p53 WT overexpression after implantation into immunocompetent syngeneic mice. 4T1 cells were implanted into female BALB/c mice for in vivo studies. Furthermore, chromatin immunoprecipitation assays indicated that p53 is recruited to the AKR1B1 promoter. We engineered p53 null 4T1 breast cancer cell line to inducibly express WTp53 and we observed that WTp53 expression increased TBK1 substrate phosphorylation (Figure 1C). Oridonin's effects on the Challenge with Cl-66 revealed that immunization with irradiated 4T1-Flt3L-p53 cells significantly slowed growth, prolonged survival, and resulted in complete remissions. Results: Delivery of p53 protein into p53-null TNBC 4T1 cells via Pos3Aa-p53 crystals restored the p53 activity, and therefore led to the induction of ICD, activation of type I IFN signaling and upregulation of PD-L1 expression. Tan IIA is potentially effective for the treatment of 4T1 breast cancer, and the molecular mechanism may be through enhancing the activity of p53 and decreasing Bcl-2 to suppress proliferation and promote apoptosis. rn7h wtduffw hz3 nc h4 il9 zh8pf uwafzu gx4l3m ml5